Acyl specificity in triglyceride synthesis by lactating rat mammary gland
نویسندگان
چکیده
منابع مشابه
Acyl specificity in triglyceride synthesis by lactating rat mammary gland.
We have studied the specificity of the acyl-CoA:diglyceride acyltransferase reaction in lactating rat mammary gland to provide a rational explanation at the enzyme level for the nonrandom distribution of fatty acids in milk fat triglycerides. Acyl-CoA:diglyceride acyltransferase activity was measured using various diglyceride and radioactive acyl-CoA substrates; products were identified as trig...
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Because vitamin A in milk is largely present as esterified retinol while blood plasma predominantly contains unesterified retinol, experiments were conducted to determine whether membranes from the lactating mammary gland are able to synthesize retinyl esters in vitro. When microsomes from rats lactating for 7 to 14 days were incubated with [(3)H]retinol dispersed in dimethylsulfoxide, some [(3...
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Fatty acid synthetase has been purified from lactating rat mammary gland and some of its physical and kinetic properties studied. This synthetase is an asymmetrical multienzyme complex of molecular weight 478,000 which readily dissociates into approximately half-molecular weight subunits. The sedimentation and diffusion coefficients of the native enzyme are 12.9 set X lo-l3 and 2.56 cm2 se@ X l...
متن کاملFatty acid synthase from lactating rat mammary gland.
Homogeneous preparations of fatty acid synthetase from mammary glands of lactating rats were used to prepare an antiserum and a y-globulin fraction was isolated from the antiserum. The antiserum and the y-globulin fraction were shown to be specific against the fatty acid synthetase multienzyme complex. Addition of the y-globulin to homogenate fractions of lactating rat mammary glsnds, resulted ...
متن کاملFunction of arginase in lactating mammary gland.
The potential for a considerable formation of ornithine exists in lactating mammary gland because of its arginase content. Late in lactation arginase reaches an activity in the gland higher than that present in any rat tissue except liver. Occurrence of the urea cycle can be excluded since two enzymes for the further reaction of ornithine in the cycle, carbamoyl phosphate synthetase I and ornit...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1976
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)41737-7